题目：Volumetric imaging in scattering media with two-photon excitation and scanned Bessel beams

时间：6月27日（周六）上午8:30

地点：光电工程学院346会议室

报告人：叶彤 助理教授（克莱姆森大学）

主持人：屈军乐 教授

报告摘要：In my talk, I will introduce a new volumetric imaging method that we have been developing in recent years. This method uses the principle of stereoscopy and the extended depth of field (EDOF). To demonstrate the method, we use Bessel beams to replace the commonly used Gaussian beams in two-photon excitation fluorescence microscopy. The EDOF of the Bessel beam and two-photon excitation provides an efficient way to acquire volumetric images of scattering samples. We have also implemented a novel scanner and written related scanning control software so that 3D stereoscopic imaging can be performedwith tilted Bessel beams. This design has dramatically improved focusing qualities and imaging speed so that the two-photon fluorescence stereomicroscopy (TPFSM) can be performed potentially in real-time to provide 3D visualization in scattering media without post image processing.

KEYWORDS: stereomicroscopy, two photon excitation, Bessel beams, extended depth of field, volumetric imaging